Department for the Study and Research of Salivary Biomarkers (DERBS)

The Département d'Étude et de Recherche de Biomarqueurs Salivaires , co-directed by Prof. Deville de Périère and Prof. Hirtz, is the result of scientific partnerships between UFR d'Odontologie teaching researchers and the Laboratoire de Biochimie et Protéomique Clinique (LBPC, CHU) and the Plateforme de Protéomique Clinique at Montpellier University Hospital. The Laboratoire de Biochimie et Protéomique Clinique (LBPC) in Montpellier is a laboratory specializing in the assay of clinical biomarkers in neuropathology and proteomics research. It is directed by Prof. Sylvain Lehmann and located within the Institut de Recherche en Médecine Régénératrice et Biothérapie (IRMB). The LBPC is home to a technology platform, the Plateforme de Protéomique Clinique (PPC), headed by Pr. Christophe Hirtz (PU Odontologie).

It was born out of the scientific continuity of the first Odontology UFR host team (EA 2988), headed by Dominique Deville de Périère and focusing on salivary physiology and neuroendocrinology.

This research department, located on the Odontology UFR site, leads clinical research projects focusing mainly on salivary biomarkers, but also more generally on biomarkers from the orofacial sphere (crevicular fluid, dental plaque, etc.). It offers nationally and internationally recognized expertise in clinical biochemical and proteomic analysis.

He was behind the creation of the first national bank of salivary samples stored at the Centre de Ressources Biologiques (CRB) at Montpellier University Hospital (Pr. S. Lehmann, Pr. D. Deville de Périère).

Biomarkers Biochemistry/proteomics

For these projects, several technological approaches based on the use of clinical samples and high analysis throughput are available (design and implementation of quantitative and qualitative mass spectrometry methods, protein identification in biological matrices, ultra-sensitive and multiplexed immunoassays).

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The PPC aims to exploit the latest technological developments in proteomics for the discovery, validation and use of biomarkers in numerous human pathologies. As part of the IBiSA-labeled Montpellier Proteome Cluster, its aim is also to make medical, biological and technical expertise in Clinical Proteomics available to academic and industrial teams. The PPC is ISO 9001-2015 certified.

Creation of the first national saliva sample bank

The DERBS team at the Faculty of Odontology, having established the first reference protocol for the conservation of salivary samples, is behind the creation of the first national bank of salivary samples and, more generally, samples from the oral cavity (crevicular fluid, dental plaque, etc.). This biobank, set up with the support of the CHU and the Centre de Ressource Biologique de Montpellier, is under the responsibility of Prof. Dominique Deville de Périère (Faculty of Odontology) and Prof. Sylvain Lehmann(CRB).

Projects

• AOI SALIVALZ (2011): Interest of salivary Abeta42 peptide assay for the diagnosis and monitoring of Alzheimer's disease
• AOI Blackstain (2016): Proteomic analysis of dental plaque in children with Black Stain.
• Parksyn (2017) : Interest of salivary alpha-synuclein assay compared to Dat-scan realization as an aid to diagnosis of idiopathic versus iatrogenic Parkinson's disease.
• BIOPARO (2017): Multiplex analysis of serum biomarkers in saliva from patients with chronic periodontitis and aggressive periodontitis by quantitative mass spectrometry (LC-MRM): a feasibility study.

Biblio

• Hirtz C, Chevalier F, Centeno D, Egea J.C, Rossignol M, Sommerer N, Deville de Périère D (2005). Complexity of the human whole saliva proteome.Journal of Physiology and Biochemistry. 61(3):469-80.

• Hirtz C, Chevalier F, Centeno D, Rofidal V, Egea J.C, Rossignol M, Sommerer N and Deville de Périère D (2005). MS characterization of multiple forms of alpha amylase in human saliva.Proteomics. 5(17):4597-607.

• Hirtz C, Chevalier F, Raingeard I, Egea JC, Sommerer N, Bringer J, Rossignol M, Deville de Périère D (2006). Salivary protein profiling in type 1 diabetes using 2-D electrophoresis and mass spectrometry.Clinical Proteomics. 2 (1-2): 117-128.

• Hirtz C, Chevalier F, Chay S, Frédéric Cuisinier, Sommerer N, Rossignol M, D Deville de Périère (2007). Proteomic Studies of Saliva: A Proposal for a Standardized Handling of Clinical Samples.Clinical Proteomics. 1559-0275-Online. 

• Morzel M, Neyraud E, Brignot H, Ducoroy P, Jeannin A, Lucchi G, Truntzer C, Canlet C, Tremblay-Franco M,Hirtz C, Gaillard S, Peretti N, Feron G. (2015) Multi-omics profiling reveals that eating difficulties developed consecutively to artificial nutrition in the neonatal period are associated to specific saliva composition.J Proteomics.128:105-12.

• Hirtz C,Vialaret J, Nowak N, Gabelle A, Deville de Périère D and Lehmann S (2016). Absolute quantification of 35 plasma biomarkers in human saliva using targeted mass spectrometry.Bioanalysis. Jan;8(1):43-53.